Influence of low-level laser irradiation on osteocalcin protein and gene expression in bone tissue1
Acta cir. bras
;
33(9): 736-743, Sept. 2018. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-973502
ABSTRACT
Abstract Purpose:
To evaluate osteocalcin gene and protein expression in vitro and in an in vivo model of ostectomy.Methods:
Twenty Wistar rats were assigned into two groups A (n=10, laser) and B (n=10, control). Ostectomy was performed in the femur diaphysis; the twenty fragments removed, composed in vitro groups named as in vivo (A and B) and cultivated in CO2 atmosphere for thirteen days. Low-level laser irradiation was performed in groups A (in vivo and in vitro) by an GaAlAs device (λ=808 nm, dose of 2J/cm2, power of 200mW, power density of 0.2W/cm2, total energy of 1.25J, spot diameter of 0.02mm) for 5 seconds, at one point, daily. It was performed immunocytochemistry assays in vivo and in vitro groups. In vitro groups were also submitted to RNA extraction, cDNA synthesis and gene expression by quantitative PCR. Statistical analysis was realized with p<0.05.Results:
Immunocytochemistry scores showed no significant differences between control and laser groups either in vivo and in vitro. Gene expression also showed no statistical differences.Conclusion:
Low-level laser irradiation did not alter osteocalcin protein and gene expression in vivo and in vitro in the studied period but it may have been expressed them in an earlier period.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Expresión Génica
/
Osteocalcina
/
Fémur
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Acta cir. bras
Asunto de la revista:
Cirugía General
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2018
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
UNIFESP/BR
/
Universidade Federal de São Paulo/BR
/
Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR
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