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Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms
Kalyani, Lella; Rao, Chava Venkata Nageswara.
  • Kalyani, Lella; NRI Institute of Technology. Department of Chemistry. Perecherla. IN
  • Rao, Chava Venkata Nageswara; NRI Institute of Technology. Department of Chemistry. Pothavarappadu. IN
Braz. J. Pharm. Sci. (Online) ; 54(3): e17258, 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-974394
ABSTRACT
A simple, accurate, isocratic stability indicating RP-HPLC method was developed for the determination of cefepime and amikacin in Pure and its pharmaceutical formulations. The method consists of methanol acetonitrileacetate buffer 752005 (v/v) mobile phase at pH 5.1 with C18 column as stationary phase. The flow rate and detection wave length were 1.0 mL/min and 212 nm respectively. The linearity range for the method was found to be 2.5-25 µg/mL for amikacin and 10-100 µg/mL cefepime respectively. The developed method was validated as per ICH guidelines and the results of all the validation parameters were well within their acceptance values. Also the forced degradation studies were conducted with standard drugs. Degradation products formed during the different stress conditions were separated from both drugs. This validated method was applied for the simultaneous estimation of cefepime and amikacin in commercially available formulation sample.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Amicacina / Cromatografía Líquida de Alta Presión / Estudio de Validación Idioma: Inglés Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2018 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: NRI Institute of Technology/IN

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Amicacina / Cromatografía Líquida de Alta Presión / Estudio de Validación Idioma: Inglés Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2018 Tipo del documento: Artículo País de afiliación: India Institución/País de afiliación: NRI Institute of Technology/IN