Inhibitory effect of reduced graphene oxide-silver nanocomposite on progression of artificial enamel caries
J. appl. oral sci
;
27: e20180042, 2019. tab, graf
Artículo
en Inglés
| LILACS, BBO
| ID: biblio-975890
ABSTRACT
Abstract The use of antimicrobial agents is an efficient method to prevent dental caries. Also, nanometric antibacterial agents with wide antibacterial spectrum and strong antibacterial effects can be applied for prevention of dental caries. Objectives:
The aim of this study was to evaluate the inhibitory effect of reduced graphene oxide-silver nanoparticles (rGO/Ag) composite on the progression of artificial enamel caries in a Streptococcus mutans biofilm model. Material andMethods:
Enamel specimens from bovine incisors were divided into eight treatment groups (n = 13), as follows group 1 was inoculated with S. mutans grown in Brain Heart Infusion containing 1% sucrose (1% BHIS), as negative control; groups 2-4 were inoculated with S. mutans grown in the presence of different rGO/Ag concentrations (0.08, 0.12, 0.16 mg/mL) + 1% BHIS; group 5-7 were inoculated with S. mutans grown in the presence of different agents (0.16 mg/mL reduced graphene oxide, 0.16 mg/mL silver nanoparticles, 10 ppm NaF) + 1% BHIS; group 8 was mixed with 1% BHIS, without inoculation. Artificial enamel carious lesions were produced by S. mutans biofilm model for 7 days. Confocal laser scanning microscopy and atomic force microscopy were used to analyze roughness and morphology of the enamel surface. Polarized light microscopy and confocal laser scanning microscopy were employed to measure the lesion depth and the relative optical density (ROD) of the demineralized layer.Results:
Compared with the control groups, the rGO/Ag groups showed (a) reduced enamel surface roughness; (b) much smoother and less eroded surfaces; (c) shallower lesion depth and less mineral loss.Conclusion:
As a novel composite material, rGO/Ag can be a promising antibacterial agent for caries prevention.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Plata
/
Streptococcus mutans
/
Caries Dental
/
Esmalte Dental
/
Nanopartículas del Metal
/
Grafito
/
Antibacterianos
Tipo de estudio:
Estudios de evaluación
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
J. appl. oral sci
Asunto de la revista:
Odontología
Año:
2019
Tipo del documento:
Artículo
País de afiliación:
China
Institución/País de afiliación:
Sun Yat-sen University/CN
/
The First Affiliated Hospital Of Hubei University Of Science And Technology/CN
Similares
MEDLINE
...
LILACS
LIS