CBS promoter hypermethylation increases the risk of hypertension and stroke
Clinics
; 74: e630, 2019. tab, graf
Article
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| ID: biblio-989638
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ABSTRACT
OBJECTIVES:
Cystathionine β-synthase is a major enzyme in the metabolism of plasma homocysteine. Hyperhomocysteinemia is positively associated with hypertension and stroke. The present study was performed to examine the possible effects of Cystathionine β-synthase promoter methylation on the development of hypertension and stroke.METHODS:
Using quantitative methylation-specific PCR, we determined the Cystathionine β-synthase methylation levels in 218 healthy individuals and 132 and 243 age- and gender-matched stroke and hypertensive patients, respectively. The relative changes in Cystathionine β-synthase promoter methylation were analyzed using the 2-ΔΔCt method. The percent of the methylated reference of Cystathionine β-synthase was used to represent the Cystathionine β-synthase promoter methylation levels.RESULTS:
In this study, the Cystathionine β-synthase promoter methylation levels of hypertensive and stroke participants were both higher than that of the healthy individuals (median percentages of the methylated reference were 50.61%, 38.05% and 30.53%, respectively, all p<0.001). Multivariable analysis showed that Cystathionine β-synthase promoter hypermethylation increased the risk of hypertension [odds ratio, OR (95% confidence interval, CI)=1.035 (1.025-1.045)] and stroke [OR (95% CI)=1.015 (1.003-1.028)]. The area under the curve of Cystathionine β-synthase promoter methylation was 0.844 (95% CI 0.796-0.892) in male patients with hypertension and 0.722 (95% CI 0.653-0.799) in male patients with stroke.CONCLUSION:
Cystathionine β-synthase promoter hypermethylation increases the risk of hypertension and stroke, especially in male patients.Palabras clave
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LILACS
Asunto principal:
Regiones Promotoras Genéticas
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Metilación de ADN
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Accidente Cerebrovascular
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Cistationina betasintasa
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Hipertensión
Tipo de estudio:
Etiology_studies
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Observational_studies
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Prognostic_studies
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Risk_factors_studies
Límite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Clinics
Asunto de la revista:
MEDICINA
Año:
2019
Tipo del documento:
Article