Occurrence, antifungal susceptibility, and virulence factors of opportunistic yeasts isolated from Brazilian beaches
Mem. Inst. Oswaldo Cruz
;
114: e180566, 2019. tab, graf
Artículo
en Inglés
| LILACS
| ID: biblio-990189
ABSTRACT
BACKGROUND Opportunistic pathogenic yeast species are frequently associated with water habitats that have pollution sources of human or animal origin. Candida albicans has already been suggested as a faecal indicator microorganism for aquatic environments. OBJECTIVES The goal of this study was to investigate the occurrence of C. albicans and other opportunistic yeasts in sand and seawater samples from beaches in Brazil to assess their correlation with Escherichia coli, and to characterise the pathogenic potential of the yeast isolates. METHODS Opportunistic species (yeasts that grow at 37ºC) were isolated from sand and seawater samples from eight beaches in Brazil during the summer and the winter. Opportunistic yeast species were evaluated for their susceptibility to antifungal drugs, virulence factors, and the in vitro and in vivo biofilm formation. Strains were selected to carry out virulence tests using BALB/c mice. FINDINGS Several water samples could be classified as inappropriate for primary contact recreation in relation to E. coli densities. C. albicans was isolated in low densities. Of the 144 opportunistic yeasts evaluated, 61% displayed resistance or dose-dependent sensitivity to at least one tested drug, and 40% produced proteinase. Strains of C. albicans and Kodamaea ohmeri exhibited the highest rates of adhesion to buccal epithelial cells. All the C. albicans strains that were tested were able to undergo morphogenesis and form a biofilm on catheter fragments in both in vitro and in vivo experiments. It was possible to confirm the pathogenic potential of three of these strains during the disseminated infection test. MAIN CONCLUSIONS The identification of opportunistic yeast species in seawater and sand samples from Brazilian beaches suggest a potential risk to the health of people who use these environments for recreational purposes.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones Oportunistas
/
Candida albicans
/
Control de Infecciones
/
Escherichia coli
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2019
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Bélgica
/
Brasil
Institución/País de afiliación:
Universidade Estadual do Paraná/BR
/
Universidade Federal de Minas Gerais/BR
/
VIB-KU Leuven Centre for Microbiology/BE
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