[ effect of adult mouse lung extracts growth factors in differentiation of umbilical cord stem cells into blood cells in vitro condition]

ABSTRACT

Stem cells [SCs] have great therapeutic indication due to their potency of self-renewal, multilineage differentiation, feasibility and safety for donors. In this study, adult mouse lung extracts containing hematopoietic growth factors were administered to umbilical cord, and evaluated the differentiation of umbilical cord stem cells into erythroid and myeloid lineages. In this basic and practical research, SCs were isolated from umbilical cord by enzyme digestion and cultured in appropriate culture medium. Subjects were divided into four groups Experimental groups 1 and 2 [E1 and E2] which were exposed to 50% and 70% concentration of lung extract for 7 days, respectively, sham [Sh] group which did not exposed to lung extract and cultured for 7 days, and control group [C]. E1, E2 and Sh groups were incubated for 7 days. All groups were evaluated by alkaline phosphatase detection kit for stem cells. Then, blood cells count and hematopoietic growth factors were assessed. ANOVA was used for data analysis. There were significant changes in E2 groups as compared with Sh and C groups, so that E2 group cells were differentiated into erythroid and myeloid lineages. Growth factors in lung extract could have stimulatory effects on umbilical cord stem cell differentiation into blood cells