[Probing the relation between gene promoters hypermethylation of RARB and p16 among patients with prostatic cancer of different prognosis]

ABSTRACT

With regard to the increasing prevalence and mortality rate of prostate cancer and several related reports studying the role of promoter Hypermethylation of RARB and p16 in predicting the prognosis, this research is carried out on 3 groups of subjects with; poor prognostic factors [case], good prognostic factors [control-2] and healthy person [control-1], at three general hospitals of Imam Hossein, Modarres and Labbafineyhad, between 2007-2009. The research has case-control Design. All three groups of participants [21 subjects in each group] were analyzed for hypermethylation of RARB- p16. All groups were matched for age, tumor stage, grade and PSA. The technique was methylation specific polymerase chain reaction [MSPCR]. In control-1, no methylation of RARB were observed. In control-2, RARB was positive in 33%. In case group, the RARB was positive for 71.4%. For control 2 and case groups, the RAR positivity was higher than control-1 [P< 0.001], however, that of case group was higher than control-2[p<0.02]. In healthy persons p16 were negative. For good prognosis group p16 were positive in 19%, while for poor prognosis one, p16 were positive in 47.6%. p16 hypermethylation was higher in case and control-2 groups [P<0.001]. Nevertheless, for case group this was higher than control-2 [P< 0.01].Hypermethylation of promotor RARB and p16 would be a good prognostic factor for early detection and prognosis of prostatic cancer