Saudi Medical Journal. 2010; 31 (6): 615-621
en Inglés
| IMEMR
| ID: emr-105244
ABSTRACT
To investigate the effect of leptin in the model of ectopic bone formation that utilizes subcutaneously implanted recombinant human bone morphogenetic protein-2 [rhBMP-2]-containing type I collagen discs. This study was performed in the Clinical Research Center, Southern Medical University, Guangzhou, China from November 2008 to June 2009. A single dose of 20 micro l saline [group A], 20 micro g leptin [group B], 2 micro g rhBMP-2 [group C], 2 micro g rhBMP-2 + 20 micro g leptin [group D], and type I collagen disks as a carrier were mixed, and subcutaneouly implanted into the back of nude mice [n=12]. The effect of ectopic bone formation was evaluated by radiography, dual energy X absorptiometry, biochemical examination of alkaline phosphatase [ALP] activity, histological observation, and semi-quantitative evaluation 4 and 8 weeks after surgery. At 4 and 8 weeks after operation the radiographs, bone mass density, ALP, and histology values showed significant intragroup and intergroup differences, with those at 8 weeks being higher than those at 4 weeks. Our results indicated that the sustainedly released material with collagen as a carrier combined treatment with leptin and rhBMP-2 has a very good osteoinductive activity. Leptin is a positive modulator for the osteoinductive efficacy of BMPs, they have synergistic effect. Its mechanisms are probably related to promote the formation of new-vessels and proliferation/ differentiation of many kinds of cells
Buscar en Google
Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Osteogénesis
/
Matriz Ósea
/
Células de la Médula Ósea
/
Factor de Crecimiento Transformador beta
/
Sistemas de Liberación de Medicamentos
/
Fosfatasa Alcalina
/
Proteína Morfogenética Ósea 2
/
Ratones Desnudos
Límite:
Animales
Idioma:
Inglés
Revista:
Saudi Med. J.
Año:
2010
Similares
MEDLINE
...
LILACS
LIS