Central role of IL-10 as key regulator of inflammation during intestinal trichinellosis in mice

ABSTRACT

This study characterized some immuno-pathological aspects of intestinal trichinellosis where, the influence of this nematode infection on levels of IL-10 mRNA expression, nitric oxide [NO] and myeloperoxidase [MPO] in intestinal mucosa is going to be studied. Four groups of albino mice were infected orally by Trichinella spiralis [T. spiralis] larvae and sacrificed on days 3, 6, 14, and 30 post infections [d.p.i.]. Levels of the previously mentioned parameters were measured in the infected intestinal tissue samples and compared to non-infected normal controls. The results showed gradual increase of IL-10 mRNA expression at 3 d.p.i., to reach its peak levels at 14 d.p.i. The mucosal levels of NO and MPO showed sharp increased by 3[rd] d.p.i., with a peak on 6 d.p.i. These biochemical changes were in accordance with the pathological changes observed by histopathological examination of stained small intestinal sections. The data reinforce the concept of the central role played by IL-10, as a Th2 cytokine in controlling severe inflammatory process initiated by NO under the influence of this invading parasite