In-Vivo and vitro studies on the effect of zinc sulphate, ascorbic acid and sodium butarate on Ehrlich Ascites carcinoma cells

ABSTRACT

The effect of zinc sulfate [ZnSO4], ascorbic acid [AsA] and sodium butarate [NaBt] were studied on the viability of Ehrlich ascites carcinoma [EAC] cells in vitro. Their antitumor effect was also studied in vivo. Eighty female Swiss albino mice were inoculated intraperitoneally with 2.5 x 106 EAC cells and injected with either 12.5 mg ZnSO4/kg body weight, 100 mg AsA/kg body weight or 100 mg NaBt/kg body weight. These compounds were injected either alone or in combinations of two or the three of them. The injection started after 24 hours for EAC inoculation and lasted for 2 days. The mean survival time [MST] as well as the change in body weights of mice of each group were determined. Another 160 female Swiss albino mice were inoculated with 2.5 x 106 EAC cells and divided into 8 groups, treated with one, two or three of the compounds under investigation, as mentioned before, and another group of 20 mice was left without any treatment serving as healthy control. Livers of mice were subjected to both biochemical and histopathological investigations