Antibody response in schistosomiasis mansoni: IgG and IgE response to three anti-schistosomal candidate vaccines glutathione -S- transferase, schistosoma mansoni 217 and schistosoma mansoni 23

ABSTRACT

A total of 199 serum samples from individuals living in a Schistosoma mansoni endemic Egyptian village were tested for their IgG and IgE response to various schistosomal antigens using ELISA. In addition, it was possible to examine the IgE response in serum samples from 54 S. mansoni infected and uninfected Sudanese subjects. IgG responses were examined against AWA, SEA, Sm GST, Sm 21.7, Sj 26, KLH and Sm 23. IgE responses were examined to these antigens as well as to a detergent extract of adult worm antigen [DAWA] and a schistosomular antigen [Somule Ag]. In the Egyptian patients studied, there was a significant negative correlation between IgG response to Sm GST intensity of infection. In male patients there was a significant difference between negative and positive IgG responses to AWA and Sm 21.7 in relation to the intensity of infection with the positive responses being associated with lower intensities of infection. However, there was no relationship between age and different IgG or IgE responses. The infected and uninfected persons did not differ significantly in their responses. In contrast, most of the IgE responses in Sudanese patients were significantly higher than those of the uninfected persons. Moreover, there was a significant negative correlation between age and IgE responses to KLH and Sm 23. Despite the contrast between the Egyptian and the Sudanese responses, the study shows an interesting pattern of antibody response to three candidate vaccines [Sm GST, Sm 21.7 and Sm 23]