effect of some inducers and inhibitors on protease production by bacillus cereus

ABSTRACT

Batch cultures of Bacillus cereus grew most luxuriantly with 3% glucose [w/v], 2% tryptone, 0.5% K2HSO4 and 1 mg/ml MnCl2H2O, supplemented to the basal medium with high enzyme activity [biomass 3.06 g/100 ml and enzyme activity 57.19 U/ml, with specific activity 39.44 U/mg protein]. All the tested nitrogen sources, ascorbic acid, ATP, chloramphenicol and vitamins exerted a significant decrease in the enzyme production