effect of somatostatin analogue, octreotide, on the jejunal mucosa of bowel resected albino rat

ABSTRACT

The aim of study was to demonstrate the effect of this drug on the histological and histochemical structure of the intestinal mucosa after small intestinal resection. 60 albino rats weighting 250 +/- 50 g were divided into 3 groups, 20 for each. Group A animals were used as controls, while the animals of groups B, C were subjected to resection of 10 cm of the jejunum. The rats of group C were also subjected to postoperative injection with sandostatin [2 mug/kg s.c. bid] for 14 days. The animals were sacrificed 2 weeks after the operation, paraffin sections from the jejunum were processed and stained with Hx, E, PAS, Grimellus technique for neuroendocrine cells and Gomori's method for alkaline phosphatase. Jejunal sections of group B showed that the mean length and breadth of jejunal villi were significantly increased with hyperplasia of the covering epithelium and marked significant increase in goblet and neuroendocrine cell populations. The brush borders of absorptive cells was more prominent with marked increase of its alkaline phosphatase activity [these changes were statistically significant as compared with group A]. Jejunal sections of group C showed that all the previous results were reversed as compared with group B [statistically significant], but insignificantly changed as compared with group A