Cloning of coagulant VII factor from hepatoma cell line

ABSTRACT

Factor VII, is a coagulant protease; it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, [rhFVII] is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line. RNA was extracted from the hepatoma, [HepG2], cell line. On reverse transcription FVII cDNA was amplified by RT-PCR. PCR product was cloned into the pTZ57R/T vector and transported into the E-coli cells. By amplification of the FVII gene, the PCR band was observed and cloning into the vector was confirmed by restriction analysis. In this paper we report the cloning of factor VII from HepG2 cell line