[In vitro assessment of sealing ability of three resin-based sealers [AH26, AH Plus Jet and TG Adseal] using microbial leakage test]

ABSTRACT

Complete root canal seal is one of the most important aims of root canal treatment. The aim of this study was to compare the sealing ability of three resin-based sealers [AH26, AH Plus Jet and TG Adseal] against microbial microleakage. In this in vitro study, 87 single-rooted extracted human teeth were decoronated maintaining a root length of 15 mm. Apical preparation and coronal flaring of the root canals were carried out up to #40 and #80 K-files, respectively, using the step-back technique. After cleaning and shaping, the teeth were randomly divided to 5 groups Three experimental groups of 25 teeth, a positive control group of 3 teeth and a negative control group of 9 teeth. The experimental groups were obturated with gutta-percha and AH26 sealer, gutta-percha and AH Plus Jet sealer, and gutta-percha and TG Adseal sealer. The samples were evaluated daily for 90 days and the time of culture contamination with Enterococcus faecalis was registered in each case. The results were statistically analyzed by Kaplan-Meier, Chi-square and Mann-Whitney tests. All the samples in the positive control group were infected after 24 hours. None of the negative control samples were infected after 90 days. Time of contamination between experimental groups showed no significant differences [p value = 0.611]. TG Adseal can be recommended for root canal therapy due to its sealability which is comparable to AH26 and AH Plus Jet root canal sealers and its appropriate cost