Inhibitory effect of gundelia extract on urinary alpha-amylase activity of type-I diabetes mellitus

ABSTRACT

alpha-amylase is an enzyme that degrades starch into maltose and glucose by hydrolyzing alpha-1,4-glucan bonds. It is known that the enzyme is found to be elevated in patients suffering from diabetes mellitus. A potent extracts of locally distributed wild plant, the Gundelia was found to inhibit elevated activity of alpha-amylase with dose responses. Samples of 50 diabetes mellitus type-I has been investigated for urinary alpha- amylase activity. An inhibitory dose of Gundelia extract were used for the inhibition of the elevated enzyme activity. The data obtained were compared with that of healthy controlled samples [30].The adopted protocol was a colorimetric determination[Anonymous,1980]. alpha-amylase activity was found to be elevated[281.70 +/- 10.03 IU/24hr] in patient's serum compared with that of controlled sample [43.38 +/- 3.33 IU/24hr]. The activity of the enzyme was found to be inhibited [160.11 +/- 250.4 IU/hr] in patients serum using dose response of 15mg/ml of Gundelia extract. The study were comprehensive to determined the physical parameters of the enzyme activity and a values of Vmax and Km were obtained. It was known that Gundelia is used in prevention and treatment of liver diseases. The plant has a role in the body as an antioxidant factor. It has a hypolipemic effect, therefore, a use of such plant extract could have a hypoglycemic activities on patients with DM-I