study of the effect of HESA-A on the wound healing process in rats

ABSTRACT

Wound healing is the restoration of physical integrity of internal and external structures and involves intricate interactions between the cells and numerous other factors. Appropriate treatment and care are essential for acceleration of the healing process, prevention from infection and chronicity of the wound; in addition different means and approaches have been used to this end. The aim of this study was to evaluate the effect of HESA-A [a drug of marine-plant origin with active biological ingredients patented by Dr. Ahmadi] on the wound healing process. The effect of HESA-A; on the 35 mm long full thickness wound in the paravertebral area 1,5mm from the midline on the back of rats. Applying a concentration of 2.5% [mixture of 2.5% drug and 97.5% chow] and 5% and 10% on the healing process. The results were evaluated measuring the length and area of the healed region on different days to conduct tensiometry experiments after complete wound healing. The percentage of wound healing on days 10, 12, 14, 16 and 18 in the control group changed in the group treated with 2.5% HESA-A from 51.27%, 62.54%, 73.11%, 86.71% and 100% to 59.34%, 75.53%, 91.17% and 100%, respectively; in the group treated with 5% HESA-A to 77.53%, 88.27%, 95.58%, 100% and 100%, respectively; and in the group treated with 10% HESA-A to 67.81%, 92.81%, 100%, 100%, and 100%, respectively. Stress [maximum tensile force causing skin rupture] changed from 16.54 in the control group to 19.2 Newton [P<0.001], 24.23 Newton [P<0.001], and 32.12 Newton [P<0.001] in the groups treated with 2.5%, 5%, and 10% HESA-A, respectively. Strain [tissue length under maximum strain] increased from 14.83 mm in the control group to 16.44 mm, 25.25 mm [P<0.001] and 35.96 mm [P<0.001] in the groups treated with 2.5%, 5%, and 10% HESA-A, respectively. Our findings suggest that HESA-A may have accelerated the skin wound healing process in rat in a concentration- dependent fashion and increased tissue strength through stimulating collagen formation