Radioprotective role of methycobal on perkinje cells of young albino rats

ABSTRACT

Therapeutic radiation used for the tumors of brain and head and neck, may damage normal brain tissue Cerebellum also shows disturbances in Its function and architecture The present experimental study was designed to observe histological changes produced by radiation in the purkinje cells of cerebellum. A prospective experimental study Place Anatomy Department Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre. Karachi. October 2008-November 2008. 30 Albino litters [day 01] were selected and divided in three groups Group "A" served as control, group "B" is radiated group and group "C" as treated group. Group B and C received radiation at the dose of 3Gy [Gray] for 202 mm in the field size of 15 x15 cm on clay 08 Group C received injection Methycobal 200 meg / kg body wt. [ip] daily from the day of radiation Animals were sacrificed on day 15.3 micro thick paraffin embedded sections of cerebellar tissue were made and stained with haematoxylin and eosin for Morphometric study of Purkinje cell. Methycobal induces the protective effects on the distorted size and monolayer of purkinje cells. The results are indicative of ameliorating effects ot Methycobal on irradiated purkinje cells of cerebellum