[Comparative study between some antioxidants on bio indices in rheumatoid arthritis induced in rats]

ABSTRACT

Rat collagen II-induced arthritis is a model of chronic inflammation induced by Mycobacterium butyricum and collagen II. It is characterized by similar pathophysiological and pathobiochemical changes as rheumatoid arthritis [RA] in humans. In the present study, the biochemical and histopathological effects of vitamins C, and aqueous extract of green tea [GTE] on RA of rats were investigated. The oxidative stress indices and prostaglandin E were evaluated. Forty male rats were divided into four groups [10 rats each] control group, collagen II-induced RA group [C II group], CII group treated with vitamin C [C II+ Vit. C], and CII group treated with green tea extract [GTE] [C II+ GTE]. After 6 weeks of antioxidants treatment, the plasma levels of lipid peroxides [LPO], nitric oxide [NO], superoxide dismutase [SOD], uric acid [UA] and glutathione [GSH] were detected using colorimetric methods. The plasma levels of prostaglandins E2 [PGE2] were measured using ELISA assay. In C II treated group, the levels of LPO, NO, PGE2, and UA, were significantly higher but the levels of SOD, and GSH were significantly lower than controls. The levels of SOD, and GSH were significantly increased but the levels of PGE2, and NO were significantly decreased in the vitamin C treated group. In the C II+ GTE group, the levels of PGE2, LPO, and NO were significantly decreased but the levels of GSH, and SOD were significantly increased in comparison with C II-treated group. The histological changes were comparable among different groups. our study suggests proper antioxidant intake management may reduce free radical generation and improve antioxidant status in RA. GTE, and vitamins C may effectively normalize in different degrees the impaired the oxidant/ antioxidant system and may be useful in delaying the complication of RA. Moreover, these antioxidants display anti-inflammatory action by alleviating foot swelling and decreasing PGE2 level in RA