Modulatory effects of garlic oil and silymarin on carbon tetrachioride-induced liver injury in rats

ABSTRACT

The present study was conducted to investigate the modulatory effects of silymarin and garlic oil on carbon tetrachloride [CCI4]-induced hepatotoxicity in male albino rats. Animals were orally intoxicated with CCI[4], after 72 hours liver toxicity was assessed. Oral administration with silym4rin or garlic was then continued for 60 days. Serum alanine transaminase [ALT], aspartate transaminase [AST], gamma-glutamyl transaminase[GGT], superoxide dismutase SOD], catalase [CAT], advanced oxidation protein product [AOPP], urinary F2 isoprostanes and 8 hydroxy 2-deoxyguanosine [8-OHdG] were measured. Intoxication of rats with CCI4 induced significant elevation in serum liver enzymes. It also increased oxidative stress through the increase in F2 isoprostanes, AOPP and 8-OHdG, but it reduced SOD and CAT as compared to that of the controls. Oral administration of silymarin or garlic oil improved these adverse effects. Silymarin or garlic has the ability to suppress the occurrence of CCl[4] induced hepatotoxicity in rats by alleviating oxidant status