Management of phenylhydrazine-induced hemolytic anemia by some antioxidants

ABSTRACT

Oxidative stress is a major contributor to the development of vascular dysfunction found in various pathological conditions. The aim of this work was to investigate the efficacy of treatment with four antioxidant drugs [Legalon, Silymarin Plus, Selenium ACE and Hipamax Plus] in anemia model of rat induced by intraperitoneal injection of phenylhydrazine at 80 mg/kg for one day. The injection of phenylhydrazine led to a significant decrease in hemoglobin level, red blood cells count, and haematocrit percent, on the other hand there was an increase in serum iron concentration, total iron binding capacity and percent of transferrin saturation when compared to their corresponding control. After 24 hours from injection of phenylhydrazine, oral administration of four antioxidants under study to rats for 2 weeks resulted in a significant increase of hemoglobin level, red blood cells count, and haematocrit percent. There was also a decrease in serum iron concentration, total iron binding capacity and percent of transferrin saturation, when compared to the group treated with phenylhydrazine [anemic group] to be within the normal values. However Hipamax Plus was the most active one when compared to the activity of the other studied antioxidants. These findings pay attention to the clinical use of antioxidants in cases of hemolytic anemia