Monocytes and peritoneal macrophage functions in patients undergoing peritoneal dialysis

ABSTRACT

Complement C2 and lysozyme produced by cultured peritoneal macrophages from 17 patients undergoing chronic ambulatory peritoneal dilaysis in the presence of dialysis fluid showed progressively less increase with culture time indicating the presence of a macrophage's inhibitory factor in patient's dialysis fluid. There was a significant reduction of C2 production by cultured patient's moncytes in day six of culture compared with normal subjects monocytes. The addition of dialysis fluid caused more reduction of C2 levels in monocytes culture from patients and normal control subjects. Lysozyme levels produced by cultured patients monocytes showed progressively reduced levels compared with normal subjects. The addition of different concentrations of dialysis fluid or patient's serum to normal monocyte culture did not change C2 or lysozyme production