influence of fructose, glucose and sucrose on flavonolignans formation in silybum marianum callus culture

ABSTRACT

Silybum marianum has been recognized as an antihepatotoxic plant. The active constituents of Silybum marianum include a group of flavonolignans known collectively as silymarin. Slymarin production by cultured cells of Silybum marianum has already been reported. Also, it was reported that physiochemical factors can influenced silymarin production in S. marinum cell cultures. Evaluation the affect of sugar source on flavonolignan production in S. marianum callus cultures. Callus culture of S. marianum were established by transferring seedling on solidified MS medium supplemented with 1 mg/1 2, 4 dichlorophenoxy acetic acid and 0.2 mg/1 kinetin. Optimal callus were subcultured to medium containing different concentration of sugars including fructose, glucose, and sucrose. Then cultures were harvested after 28 days, dried and extracted with methanol. Quantitative analyses of flavonolignans were carried out using spectrophotometric method. Higher levels of flavonolignans accumulation were observed in cultures containing 6% of all three sugars comparing with concentrations of 3% and 1.5%. It seems flavonolignans production in callus culture of S. marianum influenced by concentration of sugars rather than of sugars type