Effect of granulocyte colony-stimulating factor on liver injury induced by CCL4: a correlation between biochemical parameters and histopathology results

ABSTRACT

To determine whether granulocyte colony-stimulating factor [G-CSF] could reverse liver damage in the model of acute liver injury induced by carbon tetrachloride [CCL4]. Prospective, using experimental animal model of acute and chronic liver injury. Babol University of Medical Sciences, Babol, Iran. We established an animal model of liver damage by administration of CCL4 [1 ml/kg, IP]. Two hours later the animals were treated with G-CSF [100 microg /kg body weight, IP]. On the 28[th] day, rats were scarified. Malondialdehyde [MDA] was determined using diagnostic kits following recommendations of manufacturer of the kits. Serial 5 micro m thick liver sections were stained with haematoxylin-eosin and Masson,s trichrome and examined. Reduction in serum albumin and total protein levels [1.24 +/- 0.16 and 3.22 +/- 0.21 g/ dl, respectively] were 2.58 +/- 0.19 and 6.82 +/- 0.30 g/dl, respectively reversed by G-CSF treatment. CCL4-induced increase in serum AST, ALT, and ALP activities and MDA and hydroxyproline levels were significantly suppressed by G-CSF treatment. G-CSF stimulates liver repair and may be clinically beneficial in restoring liver damage. There was a positive correlation [p < 0.05]between histopathological and biochemical parameters