The impact of continuous supplementation vitamin B compounds against radiation in jury in rats

ABSTRACT

The influence of treatment of rats with vitamin B-compounds [daily successive doses of 168 micro g vitamin B1; 84 micro g vitamin B2; 84 micro g vitamin B6; 84 ng vitamin B12; 504 micro g nicotinamide and 214 micro g calcium Pantothenate for a period of 4 weeks] on certain biochemical parameters was examined. The variations in glycogen content and glucose-6 phosphatase activity in the liver as well as glucose, epinephrine and norepinephrine levels in the serum were indices selected in this study. No significant changes in the levels of all the studied parameters were recorded along the experimental period after treatment. Whole body gamma irradiation of rats at fractionated doses up to 8 Gy [4 equal fractions; 2 Gy weekly] caused significant increases in the liver glycogen, 1 and 7 days post exposure to the last radiation fraction followed by significant decreases, 14 and 30 days post irradiation . The activity of liver glucose-6- phosphatase and serum glucose level significantly increased 1,7 and 14 days post irradiation followed by a significant decrease after 30 days. The level of serum epinephrine significantly increased 1 and 7 days post irradiation. Conversely, the level of norepinephrine significantly decreased 1,7, and 14 days post irradiation. In rats treated with vitamin B compounds and exposed to fractionated gamma irradiation at the same above mentioned doses, certain amelioration in the radiation induced changes in the studied parameters was observed