Spectrophotometric methods in conjunction with thin layer chromatography applied for determination of sugars content in Egyptian honey and glucose syrup

ABSTRACT

A study was performed to choose the best solvent developer and adsorbent for separation of a sugar mixture composed of glucose, fructose maltose, sucrose and ribose. Thin layer chromatographic separation of the mixture is coupled with spectrophotometric method for its determination as anthrone method, phenol-sulfuric acid method and sulfuric acid method followed by application on the liquid glucose and Egyptian honey to determine qualitatively and quantitatively the content of the sugar in both of them. The solvent developer methanol, chloroform, acetone, water in ratio [22121] and ethylacetate, acetic acid, water in ratio [632] are suitable for separation of the five sugar. On applying the spectrophotometric methods, anthrone method gives the best results among the other tried spectrophotometric methods: