Prepubertal developmental studies on the anterior pituitary lobe, thyroid and uterus of barki sheep

ABSTRACT

The growth and development of pituitary, thyroid and uterus of Barki sheep were studied during prepubertal period. These tissues were embedded in paraffin and sectioned and strained with different stains. Indirect immunofluorescent technique was made for localization of LH in the cells of pituitary glands. The following changes were observed. In the pituitary gland, the appearance of LH immunoreactivity increased from the 3rd month of age onward and rose to the highest level at the 6th month then stabilized till the 9th month, from this time onward there was a gradual decrease of its activity. In the thyroid glands, there was gradual increase of thyroid activity from day 1 till 6 month, indicated by gradual increase in number and height of follicle cells. In the uterus, the endometrial glands appeared at one month, from the first month till the 5th month the endometrial glands invaginated from the surface epithelium as straight tubules, while from the 5th month till the 9th month, the endometrial gland were coiled with wide lumen containing secretory product