Study of the effect of high doses of ethenyl estradiol on the heart ultrastructural, and histopathological study in the guinea pigs

ABSTRACT

Twenty five male mature guinea pigs were included in this study. Five were used as a control group and twenty guinea pigs were given ethenyl estradiol in a dose of 100 mcg/kg body weight. Ten animals were sacrificed after two months and the other 10 animals after 4 months. The cardiac muscle showed spacing between the fibers with perivascular cellular infiltration including neutrophils and mononuclears. Later on, thickening of the wall of the small blood vessels with swollen endothelial cells and narrowing of the lumen were noticed. Associated intravascular coagulation in the small blood vessels occurred more after 4 months. EM showed spacing between fibers, dilated smooth endoplasmic reticulum, occasional fibroblasts. Therefore, estrogen should be used with great caution and it should not be used in medicated feeds