Suitability of MRS-bile agar for the selective enumeration of mixed probiotic bacteria in presence of mesophilic lactic acid cultures and yoghurt bacteria
IJB-Iranian Journal of Biotechnology. 2012; 10 (1): 16-21
en Inglés
| IMEMR
| ID: emr-122602
ABSTRACT
Measuring the viability of probiotic microorganisms in food products using plate count methodology is a common practice due to the simplicity [ease of performance], inexpensive and routine testing characters of this method. In present study, the suitability of de man rogosa and sharpe agar [MRS] bile agar medium for the selective enumeration of mixed probiotic bacteria [Lactobacillus acidophilus LA-5, L. casei 431 and Bifidobacterium lactis BB-12] in presence of mesophilic lactic cultures [Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. Cremoris] and yoghurt bacteria [Streptococcus thermophilus and Lactobacillus delbrueckii ssp. Bulgaricus] was investigated. Yoghurt bacteria did not grow neither in presence of 0.15% nor 0.30% of bile salts, as was expected. Mesophilic lactic starters could grow at both concentrations of bile salts at all incubation temperatures except 37°C. According to these results, MRS-bile agar [0.15 bile salts] could be successfully used for selective enumeration of mixed probiotic cultures in presence of mesophilic culture and/or yoghurt bacteria when plates were incubated at 37°C for 72 h
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Bacterias
/
Yogur
/
Bifidobacterium
/
Bilis
/
Ácidos y Sales Biliares
/
Lactococcus lactis
/
Ácido Láctico
/
Agar
/
Streptococcus thermophilus
/
Lactobacillus delbrueckii
Idioma:
Inglés
Revista:
Iran. J. Biotechnol.
Año:
2012
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