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Expression of IL-17 and COX2 gene in peripheral blood leukocytes of vitiligo patients
Iranian Journal of Allergy, Asthma and Immunology. 2011; 10 (2): 81-89
en Inglés | IMEMR | ID: emr-122683
ABSTRACT
Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivotal role in disease's pathogenesis. Interleukin 17 [IL-17A] is a proinflammatory cytokine which is involved in the induction of several proinflamatory mediators such as cyclooxygenase 2 [COX2]. The aim of this study was to investigate the gene expression of IL-17 and COX2 in peripheral blood leukocytes of vitiligo's patients. Peripheral blood leukocytes from 15 patients with vitiligo and 15 healthy controls were separated using a gradient density centrifugation method. After total RNA isolation and cDNA synthesis, IL-17 and COX2 gene expression were quantified by real-time polymerase chain reaction [PCR]. There were no significant differences in IL-17 and COX2 gene expression in lymphocytes of patients with vitiligo compared with control group [p<0.05]. However there was an increased IL-17 and COX2 gene expression in neutrophils of patients compared to controls, but it did not reach statistical significance [p=0.05]. We could not find any differences in IL-17 and Cox2 gene expression between clinical diseases subtypes, sex and age. There was a significant correlation between IL-17 and COX2 genes expression in the neutrophils of patients [p=0.00, r=0.80]. Our results showed an increased expression in IL-17 and Cox-2 genes in neurophils of patients with vitiligo. This suggested that these two factors are involved in the inflammatory process. Further studies with a larger sample size might help to establish the role of these factors in the pathogenesis of diseases
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Índice: IMEMR (Mediterraneo Oriental) Asunto principal: ARN Mensajero / Linfocitos / Regulación de la Expresión Génica / Interleucina-17 / Ciclooxigenasa 2 / Leucocitos / Neutrófilos Tipo de estudio: Ensayo Clínico Controlado Límite: Adolescente / Adulto / Niño / Femenino / Humanos / Masculino Idioma: Inglés Revista: Iran. J. Allergy Asthma Immunol. Año: 2011

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Índice: IMEMR (Mediterraneo Oriental) Asunto principal: ARN Mensajero / Linfocitos / Regulación de la Expresión Génica / Interleucina-17 / Ciclooxigenasa 2 / Leucocitos / Neutrófilos Tipo de estudio: Ensayo Clínico Controlado Límite: Adolescente / Adulto / Niño / Femenino / Humanos / Masculino Idioma: Inglés Revista: Iran. J. Allergy Asthma Immunol. Año: 2011