Kinetic study of purified urease enzyme from date-palm seeds

ABSTRACT

Date seeds of Alhajere variety [from Wadi Hajer - Hadramout] was studied for urease enzyme content. Results showed the occurrence of the urease enzyme, with specific activity 598 unit /mg protein. Urease extracted from Alhajere date seeds was purified by using ammonium sulphate [40-60%] saturation and dialyzed by using 20 mM potassium phosphate buffer at pH 8. The results of urease characterization showed that the optimum pH of the enzyme activity was 8 and also the same value for the optimum pH of the enzyme stability. The optimum temperature of the enzyme activity was 40°C and that for urease stability was [40-50]°C. Kinetic studies showed that Km and Vmax values were 8.4 mmolar and 975 mmole/min respectively, using urea as a substrate