[Comparative evaluation of PCR and standard serological methods in detection of brucellosis in cattle]

ABSTRACT

Brucellosis is one of the most dangerous infectious zoonotic diseases which at present affect most areas of Iran directly or indirectly. The aim of the present study was evaluate the efficacy of PCR test in detection of brucellosis. Suspected animal sera [n=20] to brucellosis were collected from clinics and subjected to Rose Bengal and Wright Agglutination tests and to PCR analysis. While 6 samples [30%] were positive with titer 180. To confirm, all samples were subjected to PCR which revealed 5 samples of 180 and 6 samples of less than 180 were in agreement with wright test. However, 8 samples of 180 and 1 sample of less than 180 were in disagreement with wright test. The results of this study revealed that considerable percent of submitted samples were positive for brucellosis and positivity of ones were confirmed with PCR test we have shown 55% agreement between SAT [Standard Agglutrination Test] and PCR results: