Production of monoclonal antibody against human nestin
AJMB-Avicenna Journal of Medical Biotechnology. 2010; 2 (2): 69-77
en Inglés
| IMEMR
| ID: emr-123752
ABSTRACT
We have employed a peptid-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N-or O-glycosylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Péptidos
/
Polietilenglicoles
/
Ensayo de Inmunoadsorción Enzimática
/
Inmunohistoquímica
/
Western Blotting
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Citometría de Flujo
/
Hibridomas
/
Proteínas de Filamentos Intermediarios
/
Proteínas del Tejido Nervioso
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Avicenna J. Med. Biotechnol.
Año:
2010
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