Molecular characterizaton of mycoplasma gallisepticum isolated from chicken and turkey

ABSTRACT

Mycoplasma gallisepticum [MG] infection in chicken and turkey is still one of the important reasons causing economic losses in poultry. The current study concerned with rapid detection and molecular characterization of MG isolates. The all samples positive by culture were positive by PCR and rt- PCR. Five isolated [four from chicken and one from turkey] were sequenced for mgc2 gene. The present molecular study proved that four wild-type MG strains. [Eis 3- C-10, Eis 4- C-10, Eis 5- C-10 were recovered from chicken and one [Eis 6-T-10] was recovered from turkey. While Eis 7-C-10 [vaccinal F-strain] was isolated from commercial layer flock vaccinated with F- strain vaccine. We concluded that mgc2 gene was able to distinguish between MG wild - type and vaccinal strains