Cloning and expression of gumboro VP2 antigen in Aspergillus niger
AJMB-Avicenna Journal of Medical Biotechnology. 2013; 5 (1): 35-41
en Inglés
| IMEMR
| ID: emr-127554
ABSTRACT
Infectious Bursal Disease Virus [IBDV] causes a highly immunosuppressive disease in chickens and is a pathogen of major economic importance to the poultry industry worldwide. The VP2 protein is the major host-protective immunogen of IBDV and has been considered as a potential subunit vaccine against the disease. VP2 coding sequence was cloned in an inducible fungal vector and the protein was expressed in Aspergillus niger [A. niger]. Aiming at a high level of expression, a multicopy AMA1-pyrG-based episomal construct driven by a strong inducible promoter, glaA, was prepared and used in transformation of A. niger pyrG[-] protoplasts. SDS-PAGE and western blot analysis was carried out to confirm the expression of the protein. A number of pyrG[+] positive transformants were isolated and the presence of expression cassette was confirmed. Western blot analysis of one of these recombinant strains using monospecific anti-VP2 antibodies demonstrated the successful expression of the protein. The recombinant protein was also detected by serum obtained from immunized chicken. In the present study, we have generated a recombinant A. niger strain expressing VP2 protein intracellulary. This recombinant strain of A. niger may have potential applications in oral vaccination against IBDV in poultry industry
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Proteínas Recombinantes
/
Virus de la Enfermedad Infecciosa de la Bolsa
Límite:
Animales
Idioma:
Inglés
Revista:
Avicenna J. Med. Biotechnol.
Año:
2013
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