Study on association between H-ras gene polymorphism and gastric adenocarcinoma risk

ABSTRACT

The aim of this study was to investigate relation between H-ras T81C polymorphism and some of the important risk factors in gastric adenocarcinoma [GA]. GA is one of the leading causes of cancer death in most countries. RAS gene is an important member in the PI3K-AKT signaling and the single nucleotide polymorphism at H-rasc DNA position 81 has been demonstrated has an important role in tumor genesis. In this study, we carried out single-nucleotide polymorphism analysis in an Iranian population. A total of 100 patients with gastric adenocarcinoma and 100 controls were examined for the presence of T81C H-ras polymorphism using PCR- RFLP assay. Statistical analysis revealed no relationship significant between TT, TC, CC and risk of GA, but, there was a poorly relation between male patient with C-carrier genotype and increasing risk of GA [P=0.07]. Also, we investigate effect of four important risk factors for GA. There was a statistically significant difference between increasing of age and susceptibility for GA [OR-1.106, 95%CI=1.073-1.139, P<0.001]. We observed a statistically significant between smoking and T81C polymorphism C-carrier genotypes [ORX3.98, 95%CI=1.831-8.68, P<0.001] as this individual had three-time risk for GA. We did not show a significant association between three main genotypes and H. pylori infection for risk of GA. These results suggested that there is no relationship between T81C-HRAS polymorphism and gastric cancer risk in Iranian patients. But, gender [male in our study] and the other risk factor described above have an important role in developing of GA