Plasminogen activators and matrix metalloproteinase activities in experimental bladder carcinogenesis

ABSTRACT

To evaluate whether or not the organophosphorus dimethoate insecticide play a vital role in the high incidence of damage of bladder organ in experimental animals. [2] To investigate the possible promoting or synergistic effects of organophosphorus compounds on the potent carcinogenic nitrosamines. [3] To protect the bladder organ from the damaging effect of either organophosphorus or nitrosaniines. Quantitative zymography was used to estimate plasminogen activators [PAs] and matrix metalloproteinases [MMPs] in male wistar albino rats urinary bladder after treatment with dimethoate in the presence and absence of diethyl nitrosoamine[DEN]. High level expressions of PAs and MMPs have been correlated with urinary bladder hyperplasia and tumors. PAs activity was significantly [p<0.05] increased in DEN initiation and dimethoate promotion in urinary bladder. MMPs expressed significant elevation [p<0.01] after the initiation with DEN and the treatment with dimethoate. Caproic acid has no significant effect throughout the experiments. Dimethoate is not carcinogenic hut it acts as promoter. PAs and MMPs may he important biochemical factors affecting urinary bladder carcinogenesis