Evaluation of cytological alterations of oral mucosa in smokers and waterpipe users

Oral mucosal epithelia of smokers and waterpipe users are more susceptible to malignant alterations. The aim of this study was morphometric evaluation of the effects of using waterpipe on normal oral mucosa. In a cross sectional study, cytologic smear samples from the following three different areas: buccal mucosa, lateral surface of the tongue, and floor of the mouth [right] were taken from 40 smokers, 40 waterpipe users, and 40 normal individuals. They were then stained using Papanicolaou staining technique. Quantitative cytologic alterations such as nuclear and cytoplasmic size, nuclear-cytoplasmic [N/C] ratio, Feret ratio [FR], percent of karriorhexis, vacuolization of cytoplasm, two or multilobed nuclei, inflammation, and candida were evaluated. Quantitative evaluation was performed using Motic Plus 2 software, and 50 cells in each slide were studied. Practitioners were matched with age and sex in three groups An increase in nuclear size, the N/C ratio, and F. R, while a decrease in cytoplasm size were observed in lateral surface of the tongue, buccal mucosa and floor of the mouth of smokers, waterpipe users and normal individuals, respectively [p