Sensitivity of crude and purified fasciola antigens in immunological diagnosis of human fascioliasis

ABSTRACT

A study has been done to find the effect of purification of crude antigens, extracted from adult Fasciola gigantica and Fasciola hepatica worms on the sensitivity of some serological techniques in diagnosis of fasciolosis. Using CIEP test, it was found that the partially purified antigens of both Fasciola species were less sensitive than their corresponding crude ones. Using IHA, it was revealed that the difference between results of crude and partially purified antigens for the two species were statistically insignificant. So, in applying this test, there is no need for the partially purification of antigen. By using ELISA, the partially purified antigens showed higher sensitivities for detection of fascioliasis than their corresponding crude ones. Partially purified F. gigantica and F. hepatica antigens gave nearly the same results whether using CIEP, IHA or ELISA. However, ELISA was the most sensitive technique followed by IHA, then CIEP