Development and implication of a capillary electrophoresis methodology for ciprofloxacin, paracetamol and diclofenac sodium in pharmaceutical formulations and simultaneously in human urine samples
Pakistan Journal of Pharmaceutical Sciences. 2011; 24 (4): 539-544
en Inglés
| IMEMR
| ID: emr-137557
ABSTRACT
This work studies the development of a simple and fairly rapid methodology for simultaneous determination/ separation of three frequently co-administered drugs; ciprofloxacin [CIP], paracetamol [PCT] and diclofenac sodium [DIG] using capillary electrophoresis [CE] with UV detection at 260 nm. Separation was achieved in only 6.5 min with a simple buffer of sodium tetraborate [50 mM] at pH 9.0. The Parameters affecting the separation and detection were optimized. The calibration curves were linear in the range of 5-500 microg/mL for CIP, 5-250 micro g/mL for PCT and 1-125 microg/mL for DIC sodium under the optimized conditions. The lower limit of detection [LOD] was found to be 1 microg/mL for CIP and PCT and 0.5 microg/mL for DIC. The method was successfully used for the analysis of drugs in commercial pharmaceutical formulations and simultaneously from patient's urine sample with RSD 0.5-2.4%. Results obtained with CE method are compared with standard HPLC procedure and were found in good agreement
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Espectrofotometría Ultravioleta
/
Comprimidos
/
Factores de Tiempo
/
Tampones (Química)
/
Calibración
/
Ciprofloxacina
/
Diclofenaco
/
Reproducibilidad de los Resultados
/
Electroforesis Capilar
/
Concentración de Iones de Hidrógeno
Límite:
Humanos
Idioma:
Inglés
Revista:
Pak. J. Pharm. Sci.
Año:
2011
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