Optimization of surfactin production by Bacillus subtilis ATCC 6633 in a miniaturized bioreactor
International Journal of Environmental Research. 2013; 7 (4): 851-858
en Inglés
| IMEMR
| ID: emr-138318
ABSTRACT
In recent years, biosurfactants due to wide applications in chemical, petroleum, food and pharmaceutical industries, have been widely considered by researchers. Biosurfactants are produced by a series of microorganisms, so it is important to screen culture medium and operating conditions in miniaturized bioreactors prior to scaling up to large bioreactors.In this study, using a kind of miniaturized bioreactor called ventilation flask, optimal production conditions, including filling volume and shaking frequency to produce a surfactin-type biosurfactant by Bacillus subtilis ATCC 6633, were examined. Moreover, the effect of oxygen transfer rate [OTR] on the surfactin production was investigated according to Amoabediny and Buchs model. The results indicated that the maximum biomass and biosurfactant yield which obtained under optimal conditions [filling volume of 15 mL and shaking frequency of 300 rpm] were evaluated 0.3 g/L/h and 0.0485 g/L/h, respectively. Also, at the same conditions, the amount of surface tension decreased from 60.5 mN/m to 31.7 mN/m and the maximum oxygen transfer rate [OTR[max]] obtained as 0.01 mol/L/h
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Polisacáridos Bacterianos
/
Bacillus subtilis
/
Reactores Biológicos
/
Lipopéptidos
Idioma:
Inglés
Revista:
Int. J. Environ. Res.
Año:
2013
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