JMB-Journal of Medical Bacteriology. 2012; 1 (3,4): 10-16
en Inglés
| IMEMR
| ID: emr-139761
ABSTRACT
The prevalence of Urinary Tract Infection [UTI] is really high in the world. Escherichia coli is a major agent of UTI. One of the strategies for decreasing UTI infections is vaccine development. As the attachment is a really important stage in colonization and infection, attachment inhibition has an applied strategy. FimH protein is a major factor during bacterial colonization in urinary tract and could be used as a vaccine. Thus, it was considered in this research as a candidate antigen. The sequences of fimH and acmA genes were used for designing a synthetic gene. It was cloned to pET23a expression vector and transformed to E. coli [DE3] Origami. To confirm the expression of recombinant protein, SDS-PAGE and western blotting methods were used. Subsequently, recombinant protein was purified. On the other hand, Lactobacillus reuteri was cultured and mixed with FimH / AcmA recombinant protein. The rate of protein localization on lactobacillus surface was assessed using ELISA method. It was showed that the recombinant protein was expressed in E. coli [DE3] Origami and purified by affinity chromatography. Moreover, this protein could be localized on lactobacillus surface by 5 days. In current study, a fusion recombinant protein was prepared and displayed on L. reuteri surface. This strain could be used for animal experiment as a competitor against Uropathogenic E. coli [UPEC]. Using manipulated probiotics strains instead of antibiotic therapy could decrease the antibiotic consumption and reduce multi-drug resistant strains
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Infecciones Urinarias
/
Ensayo de Inmunoadsorción Enzimática
/
Probióticos
/
Escherichia coli
/
Limosilactobacillus reuteri
/
Antiinfecciosos Urinarios
Idioma:
Inglés
Revista:
J. Med. Bacteriol.
Año:
2012
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