[Comparison and evaluation of proteinuria in 8-and 24 hour urine samples in patients with diabetic nephropathy]

ABSTRACT

Diabetic nephropathy is the most important disease which often results in renal failure. Proteinuria is an important parameter which predicts progression of diabetic nephropathy. Gold standard method for protein measuring is collecting 24 hour urine. Instead of that, in several studies around the world, measuring level of urine protein in 24 hours of the day has been carried out. In this study, we intended to specify the relation between quantitative amount of 8 hour and 24 hour urine protein in patients with diabetic nephropathy. The study population included 50 patients with diabetic nephropathy before ESRD We obtained the following samples 1] 8-h urine, and 2] 24-h urine. We measured serum creatinine concentration, creatinine clearance, urine volume, urinary protein and creatinine concentration. Our statistic method of analysis was Pierson's correlation method. Age mean was 53 +/- 14 years old and diabetes duration mean was 13 +/- 4.10 years. The mean of nocturnal 8hr urine protein and 24 hr one were 24.20 +/- 42, 33.35 +/- 43, 71.26 +/- 84 mg, respectively. There was a direct significant correlation between 8hr and 24hr urine protein [r=0.8, p=0.001]. A significant correlation was also detected between nocturnal 8hr urine protein and daily one with 24hr urine protein. There was a significant and direct relationship between 8hr urine protein [daily and nightly] and 24hr urine protein. This might be used for urine protein collection in patients with diabetic nephropathy to improve the compliance