NKH477 inhibits proliferation and induces apoptosis in a panel of cancer cell lines

ABSTRACT

The anti-proliferative effects of cAMP in cancer cells may be regulated by the adenylyl-cyclase-V isoform. As Colforsin Daropate [NKH477] is more selective for this isoform, we hypothesized that this water soluble compound may promise utility as an oral anti-tumour agent. Using separate cancer cell lines [MCF7, HT29, A431, WiDr, RKO, A375, H630, Du145, SW480 and SW620], we studied the effects of NKH477 on cell proliferation, cell viability and apoptosis. NKH477 induced >70% inhibition of proliferation in all cancer cell lines tested. NKH477 induced a dose-dependent apoptosis causing G1 arrest and priming cells to die. NKH477 treatment on the tested cell lines inhibited proliferation and induced apoptosis. Thus, NKH477 shows early promise as an oral anti-cancer agent