Autoreactive lymphocytotoxic antibodies in sensitised ureamic patients

We have studied 60 end stage renal disease patients, their age ranged between 2040 years. The sera of these patients were screened for the presence of lymphocytotoxic antidodies against peripheral blood lymphocytes of a random cell panel. Lymohocytotoxic antibodies were demonstrated in 25 patients [40%]. Those patient, with positive lymphocytotoxic antibodies were divided into groups. Those who undergoing maintenance heamodialysis and have recieved several blood transfusion [Group A].Those who have diagnosed for the first time and have not recieved blood [Group B]. The serum of those patients which were reactive with 30-100% of the above cell panel were then tested against autologous peripheral blood lymphocytes for detection of autoreactive lymphocytotoxic antibodies [AlcAs]. Autoreactive antibodies were detected in 50% of patients of group A and 18.8% of group B