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effect of DETA NONOate, a nitric oxide donor, on the rate of collagen synthesis in rat as an animal model of diabetes
INTJVR-International Journal of Veterinary Research. 2010; 4 (3): 159-161
en Inglés | IMEMR | ID: emr-145040
ABSTRACT
Exogenous nitric oxide donors such as DETA NONOate, spontaneously release nitric oxide. This study aimed to investigate the effect of DETA NONOate as a nitric oxide releasing drug on the rate of collagen synthesis during the impaired wound healing in a rat model of diabetes. Twelve male Sprague-Dawley rats were transferred into separate metabolic cages. Nine days before wounding, the rats were injected intraperitoneally with streptozotocin [STZ; 55 mg/kg body weight in citrate buffer 0.1 mol/L, pH 4.5] to induce diabetes. The dorsal surface of each rat was properly shaved and a full thickness dermal wound was made. The test group [n=6] was treated with 100 microM DETA NONOate in phosphate buffer while the control wounds [n=6] received sterile saline [PBS] only on the same day as wounding and every three days for one week. After the skin incision, polyvinyl alcohol [PVA] sponges were implanted subcutaneously on the dorsal of each animal under sterile conditions for the collection of wound fluid. Electrophoresis [current 20 mA] was performed on the wound fluid. The gel was stained with Coomassie blue G-250, destained, and photographed. DETA NONOate treatment increased the rate of collagen synthesis in the diabetic test group compared to the control group. The nitric oxide donor, DETA NONOate, may represent a potential treatment for impaired wound healing in diabetes by increasing the collagen synthesis at the wound site
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Índice: IMEMR (Mediterraneo Oriental) Asunto principal: Cicatrización de Heridas / Colágeno / Ratas Sprague-Dawley / Donantes de Óxido Nítrico / Modelos Animales / Diabetes Mellitus Experimental Límite: Animales Idioma: Inglés Revista: Int. J. Vet. Res. Año: 2010

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Índice: IMEMR (Mediterraneo Oriental) Asunto principal: Cicatrización de Heridas / Colágeno / Ratas Sprague-Dawley / Donantes de Óxido Nítrico / Modelos Animales / Diabetes Mellitus Experimental Límite: Animales Idioma: Inglés Revista: Int. J. Vet. Res. Año: 2010