Detection of common bacteria implicated in bovine mastitis in bulk tank milk by polymerase chain reaction

ABSTRACT

This study was conducted to detect the common bacteria implicated in bovine mastitis in bulk tank milk by polymerase chain reaction [PCR]. Forty-four milk samples from bulk tank milk were obtained and submitted to our laboratory. To detect Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Escherichia coli, Streptococcus uberis, and Streptococcus parauberis, two sets of universal primers were used. The PCR reaction was set up as described in previous literature. Using universal primers, PCR amplification results demonstrated 28 positive samples [63.6%] of 44. The percentages of positive samples for farms with production rates of < 3, 3-10, and 10 < tones were 48, 85, and 100%, respectively. In the present study, we concluded that using universal primers, a simplex PCR is able to detect common important bacteria implicated in bovine mastitis