Effects L-arginine on the gastric mucosal cell count when given with ibuprofen in albino rats under light microscope

ABSTRACT

To observe the protective effects of effects of L-Arginine on the gastric mucosal cells when given simultaneously with ibuprofen in albino rats under light microscope. A prospective experimental study. This study was conducted at the Department of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre Karachi from 01.04.2008 to 31.5.2008. This study was conducted in the Department of Anatomy, Basic Medical Sciences Institute Jinnah Postgraduate Medical Center Karachi where 45 albino rats of either sex between 90-120 days were selected and were divided into three groups, [A], [B] and [C], containing 15 animals each and were further subdivided into three sub-groups containing 5 animals, according to time of sacrifice, i.e. 4, 6, and 8 weeks respectively. Group [A] served as control. Group [B] received ibuprofen, 70 mg/kg body weight and group [C] received ibuprofen, 70mg and L-Arginine 300 mg, per kilogram body weight per day with feed. Animals were sacrificed at their respective time. The abdomen was opened, stomach was removed and opened along the greater curvature, divided into cardiac, body and pyloric parts, which in turn were fixed in Buffered neutral formalin for 24 hours. Tissues were processed in ascending strength of alcohol, cleared in xylene and infiltrated and embedded with paraffin. Five micron thick sections were made on the rotatory microtome and were stained with Haematoxylin and eosin, Periodic Acid Schiff Orange-G and Alcian blue-Periodic Acid Schiff. No abnormality was noted in gastric mucosal cell. Mean number of surface mucosal cells were decreased and results were moderately significant [P<0.001]. Mean height of surface mucous cell was increased and results were highly significant [P< 0.0001] in Bl, moderately significant [P<0.001] in B2, and significant [P< 0.05] in B3 subgroups. The mean values of mucus neck cells count was insignificant [P >0.05] when compared to control group. The mean values of chief cell count were decreased results were highly significant [P<0.0001] in Bl and B3 and significant [P< 0.05] in B2, The mean values of the parietal cells in the body were increased all these results were moderately significant [P<0.001] to highly significant [P<0.0001], when compared to control. No difference in results was noted when compared to group A. L-Arginine protects gastric mucosal cells from damage when given with ibuprofen