[GSK3beta phosphorylation with DHEA in neural progenitor cells derived from Balb/c mouse embryos brain]

ABSTRACT

Studies have shown that any disruption in wnt signaling pathway is associated with Alzheimer disease [AD]. One of the important molecules involved in activation or inactivation of this pathway is GSK3beta [glycogen syntase kinase3beta]. The main goal of this study was to evaluate GSK3beta phosphorylation by treatment of cells with dihydroepiandrosterone [DHEA], a kind of neurosteroid that decreases in the brain with aging. In this experimental study, neural progenitor cells were obtained from mouse embryos brain. Then, these cells were treated with 1microm concentration of DHEA for 48h. After 48h, the phosphorylation of GSK3beta was analyzed by immunocytochemistry. DHEA increased phosphorylation of GSK3beta in neural cells treated by DHEA, whole in control group, we could not detect the expression of GSK3beta. DHEA can increase phosphorylation of GSK3beta in neural cells and inactivation of GSK3beta can help to cure AD