Cell Journal [Yakhteh]. 2014; 16 (3): 299-308
en Inglés
| IMEMR
| ID: emr-149846
ABSTRACT
Silybin is a polyphenol with anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols can be improved by binding to phosphatidylcholine. In recent years, studies have been conducted to evaluate the anti-cancer effect of silybin. We studied the effect of silybin and silybin-phosphatidylcholine on ESR1 and ESR2 gene expression and viability in the T47D breast cancer cell line. In this experimental study, a 3-[4,5-Dimethylthiazol-2-Yl]-2,5- Diphenyltetrazolium Bromide test [MTT test] was used to determine doses for cell treatment, and the gene expression was analyzed by real-time reverse transcriptase-polymerase chain reaction [real-time RT- PCR]. Significant dose- and time-dependent cell growth inhibitory effects of silybin and silybin-phosphatidylcholine along with ESR1 down-regulation were observed in T47D cells. In contrast to ESR1, the T47D cell line showed negligible ESR2 expression. This study suggests that silybin and silybin-phosphatidylcholine down-regulate ESR1 in ER+ breast cancers. Results also show that in the T47D cell line, silybinphosphatidylcholine has a much higher growth inhibitory effect and a more significant down-regulation of ESR1 compared with silybin
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Índice:
IMEMR (Mediterraneo Oriental)
Asunto principal:
Fosfatidilcolinas
/
Neoplasias de la Mama
/
Expresión Génica
/
Línea Celular
/
Receptor alfa de Estrógeno
Límite:
Humanos
Idioma:
Inglés
Revista:
Cell J. [Yakhteh]
Año:
2014
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